LSBio
IHC-plus™ VCL / Vinculin Antibody (aa786-835)

Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ VCL / Vinculin Antibody (aa786-835)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B5770-50

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Overview

Description
Vinculin antibody LS-B5770 is an unconjugated rabbit polyclonal antibody to Vinculin (VCL) (aa786-835) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CMD1W | CMH15 | Metavinculin | VCL | Vinculin | MVCL
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P18206
SwissProt
P18206

Target

Target
VCL / Vinculin
Antigen Species
Human
Specificity
Vinculin (Ab-821) Antibody detects endogenous levels of total Vinculin protein.
Epitope
Vinculin (Ab-821) Antibody detects endogenous levels of total Vinculin protein.
Gene Name
VCL

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human Vinculin. (AA Range: 786-835)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS, 150 mM NaCl

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