LSBio
IHC-plus™ UCHL3 Antibody (C-Terminus)

Anti-UCHL3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ UCHL3 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general)
Reactivity:Bovine, Dog, Hamster, Horse, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat
Format:Liquid
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$403.00each
Catalog Number: LS-A8724-50

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Overview

Description
UCHL3 antibody LS-A8724 is an unconjugated rabbit polyclonal antibody to UCHL3 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
IHC
Reactivity
Bov, Can, Ham, Hr, Hu, Ms, NHP, Por, Rb, Rt
Predicted Reactivity
Chicken
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Ubiquitin thiolesterase | Ubiquitin thioesterase L3 | UCH-L3 | UCHL3
Recommended Dilution: IHC-P
5 - 10 µg/ml
UniProt Number
P15374
SwissProt
P15374

Target

Target
UCHL3
Antigen Species
Human
Specificity
Human UCHL3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Epitope
Human UCHL3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Gene Name
UCHL3

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic 15 amino acid peptide near C-Terminus of human UCHL3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Lizard (100%); Opossum, Turkey, Chicken (93%); Xenopus, Zebrafish, Drosophila (80%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Transgenic rescue of ataxia mice reveals a male-specific sterility defect. Crimmins S, Sutovsky M, Chen PC, Huffman A, Wheeler C, Swing DA, Roth K, Wilson J, Sutovsky P, Wilson S. Developmental biology. 2009;325:33-42.

PubMed: 18926813 · PMC: PMC2651617


Essential role of maternal UCHL1 and UCHL3 in fertilization and preimplantation embryo development. Mtango NR, Sutovsky M, Susor A, Zhong Z, Latham KE, Sutovsky P. Journal of cellular physiology. 2012 Apr;227:1592-603.

PubMed: 21678411 · PMC: PMC4351554


Essential role of ubiquitin C-terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation. Mtango NR, Sutovsky M, Vandevoort CA, Latham KE, Sutovsky P. Journal of cellular physiology. 2012 May;227:2022-9.

PubMed: 21751213 · PMC: PMC4316209


Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Yi YJ, Manandhar G, Sutovsky M, Li R, Jon[Character e1]kov[Character e1] V, Oko R, Park CS, Prather RS, Sutovsky P. Biology of reproduction. 2007 Nov;77:780-93.

PubMed: 17671268

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