Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.

IHC-plus™ TUBA1B / Tubulin Alpha 1B Antibody (C-Terminus, clone 17H11.F10)

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Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Chicken, Human, Mouse, Rat
Format:	Liquid

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$383.00each

Catalog Number: LS-B10591-50

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Overview

Description: Tubulin Alpha 1B antibody LS-B10591 is an unconjugated mouse monoclonal antibody to Tubulin Alpha 1B (TUBA1B) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IF, IHC, IHC-P, WB
Reactivity: Bov, Ck, Hu, Ms, Rt
Predicted Reactivity: Bird / Avian (Other), Bacteria, Fish (Other), Mammal (Other), Other
Nonreactive Species: Other
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Alpha-tubulin ubiquitous | Alpha tubulin | K-ALPHA-1 | TUBA1B | Tubulin, alpha 1b | Tubulin alpha-ubiquitous chain | Tubulin, alpha, ubiquitous | Tubulin K-alpha-1 | Tubulin alpha chain | Tubulin alpha-1B chain
Recommended Dilution: ELISA: 1:300000
Recommended Dilution: IF/ICC: 0.1 µg/ml
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 1:1000
UniProt Number: P68363
NCBI GenBank: NM_006082 | NP_006073.2
SwissProt: P68363

Target

Target: TUBA1B / Tubulin Alpha 1B
Antigen Species: Human
Specificity: A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Epitope: A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Gene Name: TUBA1B

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 17H11.F10
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl