LSBio
IHC-plus™ TOP2A / Topoisomerase II Alpha Antibody (N-Terminus)

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

IHC-plus™ TOP2A / Topoisomerase II Alpha Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B16084-50

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Overview

Description
Topoisomerase II Alpha antibody LS-B16084 is an unconjugated rabbit polyclonal antibody to human Topoisomerase II Alpha (TOP2A) (N-Terminus). Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IF, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DNA topoisomerase II, 170 kD | TOP2 | TOP2A | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:50
Recommended Dilution: IP
1:10 - 1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P11388
SwissProt
P11388

Target

Target
TOP2A / Topoisomerase II Alpha
Antigen Species
Human
Specificity
Recognizes endogenous levels of Topoisomerase 2 alpha protein.
Epitope
Recognizes endogenous levels of Topoisomerase 2 alpha protein.
Gene Name
TOP2A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Topoisomerase 2 alpha.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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