LSBio
IHC-plus™ TAGLN / Transgelin / SM22 Antibody (aa190-201)

Antibody staining (0.001 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

IHC-plus™ TAGLN / Transgelin / SM22 Antibody (aa190-201)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Dog, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Pig, Rat, Xenopus
Format:Liquid
See all TAGLN / Transgelin / SM22 antibodies →
$495.00each
Catalog Number: LS-B2563-50

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Overview

Description
SM22 antibody LS-B2563 is an unconjugated goat polyclonal antibody to SM22 (TAGLN / Transgelin) (aa190-201) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Bov, Can, Ham, Hr, Hu, Mml, Ms, NHP, Por, Rt, Xen
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
22 kDa actin-binding protein | SMCC | TAGLN | TAGLN1 | WS3-10 | Transgelin | Transgelin variant 2 | SM22 | Protein WS3-10 | SM22-alpha
Recommended Dilution: IHC-P
2.5 - 5 µg/ml
Recommended Dilution: WB
0.01 - 0.03 µg/ml
UniProt Number
Q01995
NCBI GenBank
NM_003186 | NP_003177.2
SwissProt
Q01995

Target

Target
TAGLN / Transgelin / SM22
Antigen Species
Human
Specificity
Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.
Epitope
Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.
Gene Name
TAGLN

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence C-MTGYGRPRQIIS, from the C-Terminus of protein sequence according to NP_003177.2.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

Proteomic profiling of chronic low-frequency stimulated fast muscle. Donoghue P, Doran P, Wynne K, Pedersen K, Dunn MJ, Ohlendieck K. Proteomics. 2007;7:3417-30.

PubMed: 17708595

Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells. Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P. Cellular and molecular life sciences : CMLS. 2009;66:1741-54.

PubMed: 19337690

Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis. Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P. Molecular biology of the cell. 2010;21:1111-24.

PubMed: 20089836 · PMC: PMC2836962

Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6. Donati C, Marseglia G, Magi A, Serrat S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P. PloS one. 2011;6:e20389.

PubMed: 21629665 · PMC: PMC3101247

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