LSBio
IHC-plus™ STAP2 Antibody (aa436-449)

Anti-STAP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

IHC-plus™ STAP2 Antibody (aa436-449)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$383.00each
Catalog Number: LS-B4920-50

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Overview

Description
STAP2 antibody LS-B4920 is an unconjugated goat polyclonal antibody to STAP2 (aa436-449) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BRK substrate | BKS | Brk kinase substrate | STAP-2 | STAP2 | Breast tumor kinase substrate
Recommended Dilution: IHC-P
3.75 µg/ml
Recommended Dilution: WB
0.5 - 1.5 µg/ml
UniProt Number
Q9UGK3
SwissProt
Q9UGK3

Target

Target
STAP2
Antigen Species
Human
Specificity
Human STAP2. This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
Epitope
Human STAP2. This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
Gene Name
STAP2

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence C-ELQKKLEKRRALEH, from the C-Terminus of protein sequence according to NP_060190.2NP_001013863.1.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T. Biochemical and biophysical research communications. 2009;384:71-5.

PubMed: 19393627


Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. Cancer science. 2011;102:756-61.

PubMed: 21205088

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