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IHC-plus™ SPRY2 / Sprouty 2 Antibody (Internal)

Immunohistochemistry of Rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: Alexa Fluoro 488 secondary at 1:500 overnight at 4C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = DAPI, red= VHL.

IHC-plus™ SPRY2 / Sprouty 2 Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B10618-50

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Overview

Description
Sprouty 2 antibody LS-B10618 is an unconjugated rabbit polyclonal antibody to human Sprouty 2 (SPRY2) (Internal). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HSPRY2 | Protein sprouty homolog 2 | SPRY2 | Sprouty homolog 2 (Drosophila) | Sprouty 2 | Spry-2 | Sprouty (Drosophila) homolog 2
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:500 - 1:5000
UniProt Number
O43597
SwissProt
O43597

Target

Target
SPRY2 / Sprouty 2
Antigen Species
Human
Specificity
Sprouty-2 antibody reacts with human Sprouty 2 protein. A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Epitope
Sprouty-2 antibody reacts with human Sprouty 2 protein. A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Gene Name
SPRY2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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