LSBio
IHC-plus™ SLIM / FHL1 Antibody (aa261-272)

Anti-FHL1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ SLIM / FHL1 Antibody (aa261-272)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Hamster, Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B2495-50

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Overview

Description
FHL1 antibody LS-B2495 is an unconjugated goat polyclonal antibody to FHL1 (SLIM) (aa261-272) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Ham, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BA535K18.1 | FHL1B | FHL1 | FHL1A | FLH1A | Four and a half LIM domains 1 | FHL-1 | KYOT | Skeletal muscle LIM-protein 1 | SLIM-1 | Skeletal muscle LIM-protein1 | SLIMMER | XMPMA | KYO-T | LIM protein SLIMMER | SLIM | SLIM1
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
0.05 - 0.3 µg/ml
UniProt Number
Q13642
SwissProt
Q13642

Target

Target
SLIM / FHL1
Antigen Species
Human
Specificity
Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Epitope
Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Gene Name
FHL1

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.2NP_001153171.1.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS
Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice. Tang T, Lai NC, Hammond HK, Roth DM, Yang Y, Guo T, Gao MH. Journal of the American College of Cardiology. 2010;55:1476-86. PubMed: 20359598 PMC: PMC3081694

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