LSBio
IHC-plus™ SLC12A2 / NKCC1 Antibody (clone 5H7)

Anti-SLC12A2 / NKCC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ SLC12A2 / NKCC1 Antibody (clone 5H7)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B6385-50

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Overview

Description
NKCC1 antibody LS-B6385 is an unconjugated mouse monoclonal antibody to human NKCC1 (SLC12A2). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Basolateral Na-K-Cl symporter | BSC2 | MBSC2 | NKCC1 | SLC12A2 | Sy-ns | BSC
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
P55011
SwissProt
P55011

Target

Target
SLC12A2 / NKCC1
Antigen Species
Human
Specificity
Human SLC12A2 / NKCC1
Epitope
Human SLC12A2 / NKCC1
Gene Name
SLC12A2

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
5H7
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
SLC12A2 (NP_001037.1, 903 aa ~ 1010 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells. Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Tatsuro Murano, Go Ito, Hiromichi Shimizu, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Akihiro Araki, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru Watanabe. Scientific reports. 2016 November;6:36795. PubMed: 27827428 PMC: PMC5101536

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