Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

IHC-plus™ SFRP1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B57-50

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Overview

Description: SFRP1 antibody LS-B57 is an unconjugated rabbit polyclonal antibody to SFRP1 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IF, IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Mouse, Rat
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: FrzA | FRP | SARP-2 | SARP2 | SFRP-1 | SFRP1 | FRP-1 | FRP1
Recommended Dilution: ELISA: 1:5000 - 1:15000
Recommended Dilution: IF/ICC: 5 µg/ml
Recommended Dilution: IHC-P: 20 µg/ml
Recommended Dilution: WB: 1:200 - 1:2000
UniProt Number: Q8N474
NCBI GenBank: NM_003012 | NP_003003.3
SwissProt: Q8N474

Target

Target: SFRP1
Antigen Species: Human
Specificity: A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Epitope: A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Gene Name: SFRP1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl