LSBio
IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal)

Anti-SDC2 / Syndecan 2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all SDC2 / Syndecan 2 antibodies →
$495.00each
Catalog Number: LS-B2981-50

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Overview

Description
Syndecan 2 antibody LS-B2981 is an unconjugated rabbit polyclonal antibody to human Syndecan 2 (SDC2) (Internal). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD362 | CD362 antigen | HSPG | HSPG1 | Fibroglycan | Syndecan proteoglycan 2 | SDC2 | SYND2 | Syndecan 2 | Syndecan-2
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
P34741
NCBI GenBank
J04621
SwissProt
P34741

Target

Target
SDC2 / Syndecan 2
Antigen Species
Human
Specificity
Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.
Epitope
Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.
Gene Name
SDC2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.25 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells. Multhaupt H, Couchman JR. Molecular cancer. Jan;14:15.

PubMed: 25623282 · PMC: PMC4326193


Effect of Repeat Dosing of Engineered Oncolytic Herpes Simplex Virus on Preclinical Models of Rhabdomyosarcoma. Waters AM, Stafman LL, Garner EF, Mruthyunjayappa S, Stewart JE, Friedman GK, Coleman JM, Markert JM, Gillespie GY, Beierle EA. Translational oncology. 2016;9:419-430.

PubMed: 27751346 · PMC: PMC5067929


N-terminal syndecan-2 domain selectively enhances 6-O heparan sulfate chains sulfation and promotes VEGFA165<sub>-dependent neovascularization. Corti F, Wang Y, Rhodes JM, Atri D, Archer-Hartmann S, Zhang J, Zhuang ZW, Chen D, Wang T, Wang Z, Azadi P, Simons M. Nature communications. 2019 April;10:1562.

PubMed: 30952866 · PMC: PMC6450910

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