LSBio
IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal)

Anti-SDC2 / Syndecan 2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2981-50

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Overview

Description
Syndecan 2 antibody LS-B2981 is an unconjugated rabbit polyclonal antibody to human Syndecan 2 (SDC2) (Internal). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD362 | CD362 antigen | HSPG | HSPG1 | Fibroglycan | Syndecan proteoglycan 2 | SDC2 | SYND2 | Syndecan 2 | Syndecan-2
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
P34741
NCBI GenBank
J04621
SwissProt
P34741

Target

Target
SDC2 / Syndecan 2
Antigen Species
Hu
Specificity
Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.
Epitope
Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.
Gene Name
SDC2

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.25 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells. Multhaupt H, Couchman JR. Molecular cancer. Jan;14:15. PubMed: 25623282 PMC: PMC4326193

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