LSBio
IHC-plus™ RNF2 / RING2 / RING1B Antibody (aa189-201)

Anti-RNF2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

IHC-plus™ RNF2 / RING2 / RING1B Antibody (aa189-201)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
See all RNF2 / RING2 / RING1B antibodies →
$526.00each
Catalog Number: LS-B417-50

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Overview

Description
RING1B antibody LS-B417 is an unconjugated goat polyclonal antibody to RING1B (RNF2 / RING2) (aa189-201) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, NHP
Predicted Reactivity
Bovine, Dog, Chicken, Mouse, Non-Human Primate, Other, Rat, Zebrafish
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DING | HIP2-interacting protein 3 | Protein DinG | Ring finger protein 2 | RNF2 | RING finger protein BAP-1 | BAP-1 | HIPI3 | RING finger protein 1B | RING1B | RING2
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: IF/ICC
1:300
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q99496
SwissProt
Q99496

Target

Target
RNF2 / RING2 / RING1B
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
Gene Name
RNF2

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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