LSBio
IHC-plus™ RNF125 / TRAC-1 Antibody (aa131-180)

Anti-RNF125 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ RNF125 / TRAC-1 Antibody (aa131-180)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$470.00each
Catalog Number: LS-B11326-50

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Overview

Description
TRAC-1 antibody LS-B11326 is an unconjugated rabbit polyclonal antibody to TRAC-1 (RNF125) (aa131-180) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
RNF125 | RING finger protein 125 | TRAC-1 | TRAC1
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q96EQ8
SwissProt
Q96EQ8

Target

Target
RNF125 / TRAC-1
Antigen Species
Human
Specificity
RNF125 Antibody detects endogenous levels of total RNF125 protein.
Epitope
RNF125 Antibody detects endogenous levels of total RNF125 protein.
Gene Name
RNF125

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human RNF125. (AA Range: 131-180)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS, 150 mM NaCl
Sequential ubiquitination of NLRP3 by RNF125 and Cbl-b limits inflammasome activation and endotoxemia. Juan Tang, Sha Tu, Guoxin Lin, Hui Guo, Chengkai Yan, Qingjun Liu, Ling Huang, Na Tang, Yizhi Xiao, R Marshall Pope, Murugesan V S Rajaram, Amal O Amer, Brian M Ahmer, John S Gunn, Daniel J Wozniak, Lijian Tao, Vincenzo Coppola, Liwen Zhang, Wallace Y Langdon, Jordi B Torrelles, Stanley Lipkowitz, Jian Zhang. The Journal of experimental medicine. 2020 April;217. PubMed: 31999304 PMC: PMC7144527

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