Anti-PPAR Alpha antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

IHC-plus™ PPARA / PPAR Alpha Antibody (aa1-25)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Dog, Hamster, Mouse, Pig, Rat
Format:	Liquid

See all PPARA / PPAR Alpha antibodies â†’

$526.00each

Catalog Number: LS-B46-50

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Quantity

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Overview

Description: PPAR Alpha antibody LS-B46 is an unconjugated rabbit polyclonal antibody to PPAR Alpha (PPARA) (aa1-25) from mouse. It is reactive with mouse, rat, boar and other species. Validated for ELISA, Flow, IF, IHC and WB. Cited in 1 publication.
Application: ELISA, FC, IF, IHC, IHC-P, WB
Reactivity: Bov, Can, Ham, Ms, Por, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: PPAR Alpha | PPARalpha | PPAR-alpha | NR1C1 | HPPAR | PPAR | PPARA
Recommended Dilution: ELISA: 1:75000 - 1:125000
Recommended Dilution: IF/ICC: 1 - 5 µg/ml
Recommended Dilution: IHC-P: 20 µg/ml
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q07869
NCBI GenBank: NM_005036 | NP_005027.2
SwissProt: Q07869

Target

Target: PPARA / PPAR Alpha
Antigen Species: Mouse
Specificity: A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Epitope: A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Gene Name: PPARA

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl

PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer. Shailender S Chauhan, Andrea L Casillas, Andres D Vizzerra, Hope Liou, Amber N Clements, Caitlyn E Flores, Christopher T Prevost, David F Kashatus, Ashley J Snider, Justin M Snider, Noel A Warfel. Oncogene. 2024 February;43:406-419. PubMed: 38097734 PMC: PMC10837079

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