LSBio
IHC-plus™ Polyclonal Rabbit AQP3 / Aquaporin 3 Antibody (aa268-292, IHC, IF, WB)

Anti-AQP3 / Aquaporin 3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Polyclonal Rabbit AQP3 / Aquaporin 3 Antibody (aa268-292, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all AQP3 / Aquaporin 3 antibodies →
$383.00each
Catalog Number: LS-B8185-50

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Overview

Description
Aquaporin 3 antibody LS-B8185 is an unconjugated rabbit polyclonal antibody to Aquaporin 3 (AQP3) (aa268-292) from human, mouse and rat. Validated for IF, IHC and WB. Cited in 4 publications.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AQP3 | Aquaporin 3 (GIL blood group) | Aquaglyceroporin-3 | AQP-3 | Aquaporin 3 | Aquaporin 3 (Gill blood group) | Aquaporin-3 | GIL
Recommended Dilution: IF/ICC
1:200 - 1:400
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000 - 1:4000
UniProt Number
Q92482
SwissProt
Q92482

Target

Target
AQP3 / Aquaporin 3
Specificity
Detects ~31.5kDa.Glycosylated bands ~35-50kDa.
Epitope
Detects ~31.5kDa.Glycosylated bands ~35-50kDa.
Gene Name
AQP3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
C-Terminus peptide (Rat)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

SOX2 regulates acinar cell development in the salivary gland. Emmerson E, May AJ, Nathan S, Cruz-Pacheco N, Lizama CO, Maliskova L, Zovein AC, Shen Y, Muench MO, Knox SM. eLife. 2017;5.

PubMed: 28623666 · PMC: PMC5498133


Expression and clinical significance of aquaglyceroporins in human hepatocellular carcinoma. Chen XF, Li CF, Lü L, Mei ZC. Molecular medicine reports. 2016 June;13:5283-9.

PubMed: 27121567


Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement. Emmerson E, May AJ, Berthoin L, Cruz-Pacheco N, Nathan S, Mattingly AJ, Chang JL, Ryan WR, Tward AD, Knox SM. EMBO molecular medicine. 2018 March;10:pii: e8051.

PubMed: 29335337 · PMC: PMC5840548


Aquaporin-3 re-expression induces differentiation in a phospholipase D2-dependent manner in aquaporin-3-knockout mouse keratinocytes. Vivek Choudhary, Lawrence O Olala, Haixia Qin, Inas Helwa, Zhi-Qiang Pan, Ying-Ying Tsai, Michael A Frohman, Ismail Kaddour-Djebbar, Wendy B Bollag. The Journal of investigative dermatology. 2015 Feb;;135:499-507.

PubMed: 25233074 · PMC: PMC7278525

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