LSBio
IHC-plus™ Polyclonal Rabbit anti-Rat HTR3A / 5-HT3A Receptor Antibody (aa21-36, IHC, WB)

Anti-5HT3A receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Rat HTR3A / 5-HT3A Receptor Antibody (aa21-36, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B2042-50

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Overview

Description
5-HT3A Receptor antibody LS-B2042 is an unconjugated rabbit polyclonal antibody to 5-HT3A Receptor (HTR3A) (aa21-36) from rat. It is reactive with human and rat. Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
5-HT-3R | 5-HT3-A | 5-HT3R-A | 5-HT3 receptor | 5HT3 serotonin receptor | 5-HT3A | 5-HT3a receptor | 5-hydroxytryptamine receptor 3 | 5-HT-3 | 5-HT3 | 5-HT3R | 5HT3R | HTR3A | HTR3 | Serotonin receptor 3A
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 µg/ml
UniProt Number
P46098
SwissProt
P46098

Target

Target
HTR3A / 5-HT3A Receptor
Antigen Species
Rat
Specificity
A mixture of synthetic peptides corresponding to amino acids 21-36 and 421-436 of rat 5-HT3 receptor
Epitope
In human brain, a 47-50 kD band is observed.
Gene Name
HTR3A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%); Mouse (88%); Hamster (81%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
HTR3A is correlated with unfavorable histology and promotes proliferation through ERK phosphorylation in lung adenocarcinoma. Mitsuyo Tone, Shinichiro Tahara, Satoshi Nojima, Daisuke Motooka, Daisuke Okuzaki, Eiichi Morii. Cancer science. 2020 October;111:3953-3961. PubMed: 32736413 PMC: PMC7540989

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