Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-CAMK2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Rat CAMK2 / CAMKII Antibody (IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Bovine, Chicken, Dog, Horse, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat, Xenopus, Zebrafish |
| Format: | Liquid |
$383.00each
Catalog Number: LS-B3743-50
Catalog Number: LS-B3743
For bulk orders or custom formulations:
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- Description
- CAMKII antibody LS-B3743 is an unconjugated rabbit polyclonal antibody to CAMKII (CAMK2) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
- Application
- IHC, IHC-P, WB
- Reactivity
- Bov, Can, Ck, Hr, Hu, Ms, NHP, Por, Rb, Rt, Xen, Zf
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:1000
Target
- Target
- CAMK2 / CAMKII
- Antigen Species
- Rat
- Specificity
- Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.
- Epitope
- Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (aa 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%); Orangutan (93%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 25 mM Sodium Borate, BBS, pH 8.2, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- 25 mM Sodium Borate, BBS, 75 mM NaCl
Role of CaMKII in Ang-II-dependent small artery remodeling. Prasad AM, Ketsawatsomkron P, Nuno DW, Koval OM, Dibbern ME, Venema AN, Sigmund CD, Lamping KG, Grumbach IM. Vascular pharmacology. 2016 December;87:172-179. PubMed: 27658984 PMC: PMC5164857
