Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-ATG5-ATG12 Complex antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Mouse ATG5-ATG12 Complex Antibody (aa262-275, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$720.00each
Catalog Number: LS-B9976-0.05
Catalog Number: LS-B9976
For bulk orders or custom formulations:
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- Description
- ATG5-ATG12 Complex antibody LS-B9976 is an unconjugated rabbit polyclonal antibody to mouse ATG5-ATG12 Complex (aa262-275). Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
Target
- Target
- ATG5-ATG12 Complex
- Antigen Species
- Mouse
- Specificity
- Specifically recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses. Studies have shown that the Atg5-Atg12 complex directly interacts with RIG-1 (retinoic acid-inducible gene 1) and the adaptor molecule IPS-1 (IFN-beta promoter stimulator 1), through caspase recruitment domains (CARDs), thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells.
- Epitope
- Recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses (Hwang et al. 2012). The Atg5-Atg12 complex interacts with retinoic acida"inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS
