LSBio
IHC-plus™ Polyclonal Rabbit anti-Mouse ANGPT1 / Angiopoietin-1 Antibody (aa15-45, IHC, WB)

Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of human lung, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ Polyclonal Rabbit anti-Mouse ANGPT1 / Angiopoietin-1 Antibody (aa15-45, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$526.00each
Catalog Number: LS-B62-50

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Overview

Description
Angiopoietin-1 antibody LS-B62 is an unconjugated rabbit polyclonal antibody to Angiopoietin-1 (ANGPT1) (aa15-45) from mouse. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Angiopoietin 1 | Angiopoietin-1 | AGPT | AGP1 | ANG-1 | ANGPT1 | ANG1 | KIAA0003
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q15389
SwissProt
Q15389

Target

Target
ANGPT1 / Angiopoietin-1
Antigen Species
Mouse
Specificity
This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Epitope
This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Gene Name
ANGPT1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
80 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels. de Vries MR, Parma L, Peters HAB, Schepers A, Hamming JF, Jukema JW, Goumans MJTH, Guo L, Finn AV, Virmani R, Ozaki CK, Quax PHA. Journal of internal medicine. 2019 January;285:59-74. PubMed: 30102798 PMC: PMC6334526

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