LSBio
IHC-plus™ Polyclonal Rabbit anti-Mammal Collagen V Antibody (Biotin, IHC, WB)

Anti-Collagen V antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Mammal Collagen V Antibody (Biotin, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Human, Mammal (Other)
Format:Liquid
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$536.00each
Catalog Number: LS-B379-50

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Overview

Description
Collagen V antibody LS-B379 is a biotin-conjugated rabbit polyclonal antibody to Collagen V from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Bov, Hu, Mml
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:4000 - 1:8000
Recommended Dilution: IHC-P
1.25 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:5000 - 1:10000

Target

Target
Collagen V
Antigen Species
Mammal (Other)
Specificity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Epitope
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Collagen Type V from human and bovine placenta.

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.