LSBio
IHC-plus™ Polyclonal Rabbit anti-Mammal Collagen IV Antibody (IHC, WB)

Anti-Collagen IV antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Mammal Collagen IV Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Human, Mammal (Other)
Format:Liquid
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$526.00each
Catalog Number: LS-B2717-50

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Overview

Description
Collagen IV antibody LS-B2717 is an unconjugated rabbit polyclonal antibody to Collagen IV from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Bov, Hu, Mml
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:50000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:1000 - 1:10000

Target

Target
Collagen IV
Antigen Species
Mammal (Other)
Specificity
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Epitope
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Collagen Type IV from human and bovine placenta.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.