LSBio
IHC-plus™ Polyclonal Rabbit anti-Human UBP43 / USP18 Antibody (IHC, WB)

Anti-USP18 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human UBP43 / USP18 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B1182-50

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Overview

Description
USP18 antibody LS-B1182 is an unconjugated rabbit polyclonal antibody to human USP18 (UBP43). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
43 kDa ISG15-specific protease | ISG43 | HUBP43 | ISG15 specific protease | Ubiquitin specific protease 18 | Ubl thioesterase 18 | Ubl thiolesterase 18 | UBP43 | USP18
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9UMW8
SwissProt
Q9UMW8

Target

Target
UBP43 / USP18
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. The core 7 amino acid sequence QPRERPR of the 10 amino acid immunogen sequence may be reactive with UBP43 from mouse and dog. This core sequence also shows partial homology with proteins other than UBP43. Cross reactivity with UBP43 homologues from other sources has not been determined.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. The core 7 amino acid sequence QPRERPR of the 10 amino acid immunogen sequence may be reactive with UBP43 from mouse and dog. This core sequence also shows partial homology with proteins other than UBP43. Cross reactivity with UBP43 homologues from other sources has not been determined.
Gene Name
USP18

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.93 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia. AbdulMajeed AA, Dalley AJ, Farah CS. Annals of diagnostic pathology. 2013 Aug;17:331-40. PubMed: 23643910

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