LSBio
IHC-plus™ Polyclonal Rabbit anti-Human SIPR3 / EDG3 / S1P3 Antibody (aa12-25, IHC, WB)

Anti-EDG3 antibody IHC of human prostate, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human SIPR3 / EDG3 / S1P3 Antibody (aa12-25, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2155-50

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Overview

Description
S1P3 antibody LS-B2155 is an unconjugated rabbit polyclonal antibody to human S1P3 (SIPR3 / EDG3) (aa12-25). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ICC, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
EDG-3 | EDG3 | LPB3 | S1P receptor EDG3 | S1P3 | S1PR3 | S1P receptor Edg-3 | S1P receptor 3
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
3 µg/ml
UniProt Number
Q99500
SwissProt
Q99500

Target

Target
SIPR3 / EDG3 / S1P3
Antigen Species
Hu
Specificity
Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
Epitope
Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
Gene Name
S1PR3

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (93%); Marmoset (86%).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
TBS, pH 7.4, 5 mg/ml BSA
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
No
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS
Sphingosine-1-phosphate receptor 3 mediates sphingosine-1-phosphate induced release of weibel-palade bodies from endothelial cells. van Hooren KW, Spijkers LJ, van Breevoort D, Fernandez-Borja M, Bierings R, van Buul JD, Alewijnse AE, Peters SL, Voorberg J. PloS one. 2014;9:e91346. PubMed: 24632890 PMC: PMC3954688

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