Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-EDG3 antibody IHC of human prostate, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Human SIPR3 / EDG3 / S1P3 Antibody (aa12-25, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$495.00each
Catalog Number: LS-B2155-50
Catalog Number: LS-B2155
For bulk orders or custom formulations:
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- Description
- S1P3 antibody LS-B2155 is an unconjugated rabbit polyclonal antibody to human S1P3 (SIPR3 / EDG3) (aa12-25). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
- Application
- ICC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EDG-3 | EDG3 | LPB3 | S1P receptor EDG3 | S1P3 | S1PR3 | S1P receptor Edg-3 | S1P receptor 3
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 3 µg/ml
- UniProt Number
- Q99500
- NCBI GenBank
- NM_005226 | NP_005217.2
- SwissProt
- Q99500
Target
- Target
- SIPR3 / EDG3 / S1P3
- Antigen Species
- Human
- Specificity
- Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
- Epitope
- Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
- Gene Name
- S1PR3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (93%); Marmoset (86%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, pH 7.4, 5 mg/ml BSA
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- TBS
Sphingosine-1-phosphate receptor 3 mediates sphingosine-1-phosphate induced release of weibel-palade bodies from endothelial cells. van Hooren KW, Spijkers LJ, van Breevoort D, Fernandez-Borja M, Bierings R, van Buul JD, Alewijnse AE, Peters SL, Voorberg J. PloS one. 2014;9:e91346.
PubMed: 24632890 · PMC: PMC3954688
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Peksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS. Nature communications. 2018 July;9:2705.
PubMed: 30006619 · PMC: PMC6045677
