LSBio
IHC-plus™ Polyclonal Rabbit anti-Human RAF1 / RAF Antibody (C-Terminus, IHC, IF, WB)

Immunohistochemical analysis of c-RAF staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

IHC-plus™ Polyclonal Rabbit anti-Human RAF1 / RAF Antibody (C-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Chicken, Human, Mouse, Rat
Format:Liquid
See all RAF1 / RAF antibodies →
$383.00each
Catalog Number: LS-B14835-50

For bulk orders or custom formulations:

Request a Quote

Overview

Description
RAF antibody LS-B14835 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IF, IHC, IHC-P, WB
Reactivity
Bov, Ck, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | RAF1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:100 - 1:200
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P04049
SwissProt
P04049

Target

Target
RAF1 / RAF
Antigen Species
Human
Specificity
Recognizes endogenous levels of c-RAF protein.
Epitope
Recognizes endogenous levels of c-RAF protein.
Gene Name
RAF1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human c-RAF.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.