Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human PRKDC / DNA-PKcs Antibody (phospho-Thr2609, IHC, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B409-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: DNA-PKcs antibody LS-B409 is an unconjugated rabbit polyclonal antibody to DNA-PKcs (PRKDC) (pThr2609) from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-P, IP, WB
Reactivity: Hu
Predicted Reactivity: NHP
Intended Use: RUO
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DNA-PK | DNAPK | DNPK1 | HYRC | HYRC1 | p350 | PRKDC | Scid | XRCC7 | DNA-PK catalytic subunit | DNA-PKcs | p460 | DNA-dependent protein kinase catalytic subunit
Recommended Dilution: ELISA: 1:300000
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P78527
NCBI GenBank: NM_006904, NP_008835.5
SwissProt: P78527

Target

Target: PRKDC / DNA-PKcs
Antigen Species: Hu
Specificity: This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. However, cross-reactivity is expected with mouse, rat, dog, chicken and horse DNA PKcs based on a high degree of homology to the immunogen sequence. Cross-reactivity with DNA PKcs homologues from other sources has not been determined.
Epitope: This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. However, cross-reactivity is expected with mouse, rat, dog, chicken and horse DNA PKcs based on a high degree of homology to the immunogen sequence. Cross-reactivity with DNA PKcs homologues from other sources has not been determined.
Gene Name: PRKDC

Antibody

Host Species: Rb
Clonality: Polyclonal
Origin Species: Hu

Immunogen

Immunogen: Rabbit Anti-DNA PKcs pT2609 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr 2609 of human DNA PKcs.

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: AP

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate