LSBio
IHC-plus™ Polyclonal Rabbit anti-Human PKG / PRKG1 Antibody (aa657-671, IHC, WB)

Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human PKG / PRKG1 Antibody (aa657-671, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Pig, Rat
Format:Liquid
See all PKG / PRKG1 antibodies →
$526.00each
Catalog Number: LS-B2219-50

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Overview

Description
PRKG1 antibody LS-B2219 is an unconjugated rabbit polyclonal antibody to PRKG1 (PKG) (aa657-671) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, Por, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CGKI | CGMP kinase I | CGMP protein kinase type I | CGK-I | CGK1 | PKGI | PKG I | PRKGR1B | PRKG1 | PRKG1B | PRKGR1A | PGK | PKG | CGK | CGK 1 | CGKI-alpha | CGKI-BETA
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1 µg/ml
UniProt Number
Q13976
SwissProt
Q13976

Target

Target
PKG / PRKG1
Antigen Species
Human
Specificity
Recognizes human PKG1 alpha at ~75kD.
Epitope
Recognizes human PKG1 alpha at ~75kD.
Gene Name
PRKG1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (aa 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Recommended Storage Buffer
PBS
Cyclic guanosine monophosphate and the dependent protein kinase regulate lymphatic contractility in rat thoracic duct. Gasheva OY, Gashev AA, Zawieja DC. The Journal of physiology. 2013 Sep;591:4549-65. PubMed: 23836689 PMC: PMC3784198

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