LSBio
IHC-plus™ Polyclonal Rabbit anti-Human PDK1 Antibody (aa350-436, IHC, WB)

Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Polyclonal Rabbit anti-Human PDK1 Antibody (aa350-436, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B1733-0.1

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Overview

Description
PDK1 antibody LS-B1733 is an unconjugated rabbit polyclonal antibody to PDK1 (aa350-436) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Predicted Reactivity
Chicken
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
PDHK | PDK1 | PDH kinase 1 | PDHK1
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
2 µg/ml
UniProt Number
Q15118
SwissProt
Q15118

Target

Target
PDK1
Antigen Species
Human
Specificity
PDK1.
Epitope
PDK1.
Gene Name
PDK1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide made to a C-Terminus portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]. Percent identity by BLAST analysis: Mouse, Rat (100%); Chicken (93%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Tris-Glycine, 150 mM NaCl
Concentration
1 mg/mL
Volume
0.1 Milliliter
Animal Free
No
Carrier-Free
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
Tris-Glycine, 150 mM NaCl
Enhanced glucose metabolism through activation of HIF-1a covers the energy demand in a rat embryonic heart primordium after heartbeat initiation. Tatsuya Sato , Nobutoshi Ichise , Takeshi Kobayashi, Hiroyori Fusagawa, Hiroya Yamazaki, Taiki Kudo, Noritsugu Tohse. Scientific reports. 2022 January;12:74. PubMed: 34996938 PMC: PMC8741773

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