Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-MYL9 antibody IHC of human smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Human MLC2 / MYL9 Antibody (IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B38-50
Catalog Number: LS-B38
For bulk orders or custom formulations:
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- Description
- MYL9 antibody LS-B38 is an unconjugated rabbit polyclonal antibody to MYL9 (MLC2) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 20 kDa myosin light chain | LC20 | MLC-2C | MLC2 | MYL9 | Myosin RLC | MRLC1 | MYRL2
- Recommended Dilution: ELISA
- 1:10000 - 1:70000
- Recommended Dilution: IHC-P
- 2.5 µg/ml
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P24844
- NCBI GenBank
- NM_006097 | NP_006088.2
- SwissProt
- P24844
Target
- Target
- MLC2 / MYL9
- Antigen Species
- Human
- Specificity
- The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- Epitope
- The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- Gene Name
- MYL9
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria. Blutke A, Block C, Berendt F, Herbach N, Kemter E, Amann K, Fr[Character f6]hlich T, Arnold GJ, Wanke R. Proteomics. Clinical applications. 2011 Jun;5:375-81. PubMed: 21538912
