LSBio
IHC-plus™ Polyclonal Rabbit anti-Human FHIT Antibody (IHC, WB)

Anti-FHIT antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human FHIT Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Rat
Format:Liquid
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$495.00each
Catalog Number: LS-B3166-50

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Overview

Description
FHIT antibody LS-B3166 is an unconjugated rabbit polyclonal antibody to FHIT from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AP3Aase | Dinucleosidetriphosphatase | FHIT | Fragile histidine triad | Fragile histidine triad gene | AP3A hydrolase | Bis(5-adenosyl)-triphosphatase | FRA3B | Tumor suppressor protein
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
P49789
SwissProt
P49789

Target

Target
FHIT
Antigen Species
Human
Specificity
Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.
Epitope
Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.
Gene Name
FHIT

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Full length human FHIT fusion protein with GST (glutathione amino transferase)
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Recommended Storage Buffer
PBS

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