LSBio
IHC-plus™ Polyclonal Rabbit anti-Human FANCF Antibody (Internal, IHC, WB)

Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human FANCF Antibody (Internal, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B210-50

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Overview

Description
FANCF antibody LS-B210 is an unconjugated rabbit polyclonal antibody to FANCF (Internal) from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Fanconi anemia group F protein | FANCF | Protein FACF | FAF
Recommended Dilution: ELISA
1:15000 - 1:60000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
Q9NPI8
SwissProt
Q9NPI8

Target

Target
FANCF
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Epitope
A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
FANCF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal amino acid sequence of human FANCF.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Development of a 3D functional assay and identification of biomarkers, predictive for response of high-grade serous ovarian cancer (HGSOC) patients to poly-ADP ribose polymerase inhibitors (PARPis): targeted therapy. Razan Sheta, Magdalena Bachvarova, Marie Plante, Marie-Claude Renaud, Alexandra Sebastianelli, Jean Gregoire, Jamilet Miranda Navarro, Ricardo Bringas Perez, Jean-Yves Masson, Dimcho Bachvarov. Journal of translational medicine. 2020 November;18:439. PubMed: 33213473 PMC: PMC7678187

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