LSBio
IHC-plus™ Polyclonal Rabbit anti-Human EN1 / Engrailed Antibody (aa1-30, IHC, IF, WB)

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ Polyclonal Rabbit anti-Human EN1 / Engrailed Antibody (aa1-30, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all EN1 / Engrailed antibodies →
$470.00each
Catalog Number: LS-B9070-100

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Overview

Description
Engrailed antibody LS-B9070 is an unconjugated rabbit polyclonal antibody to human Engrailed (EN1) (aa1-30). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Engrailed 1 | Engrailed homolog 1 | EN1 | Homeobox protein en-1 | Homeobox protein engrailed-1 | Hu-En-1 | Engrailed homeobox 1
Recommended Dilution: IF/ICC
1:10 - 1:50
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
Q05925
SwissProt
Q05925

Target

Target
EN1 / Engrailed
Antigen Species
Human
Specificity
This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Epitope
This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Gene Name
EN1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS with 0.09% (w/v) sodium azide
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal a-motoneurons. Mélanie Lebœuf , Stephanie E Vargas-Abonce , Eugénie Pezé-Hedsieck, Edmond Dupont, Lucia Jimenez-Alonso, Kenneth L Moya, Alain Prochiantz. EMBO reports. 2023 August;24:e56525. PubMed: 37534581 PMC: PMC10437418

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