LSBio
IHC-plus™ Polyclonal Rabbit anti-Human DDB1 Antibody (aa198-213, IHC, WB)

Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ Polyclonal Rabbit anti-Human DDB1 Antibody (aa198-213, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$526.00each
Catalog Number: LS-B364-50

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Overview

Description
DDB1 antibody LS-B364 is an unconjugated rabbit polyclonal antibody to DDB1 (aa198-213) from human. It is reactive with human and mouse. Validated for DB, ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
DB, ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DDB p127 subunit | DDB1 | DDBA | HBV X-associated protein 1 | UV-DDB1 | XAP-1 | XPE | XPE-binding factor | UV-damaged DNA-binding factor | XPCE | DNA damage-binding protein 1 | DNA damage-binding protein a | UV-DDB 1 | XAP1 | XPE-BF
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: IP
1:500
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q16531
SwissProt
Q16531

Target

Target
DDB1
Antigen Species
Human
Specificity
Reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.
Epitope
Reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.
Gene Name
DDB1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.

The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. Molecular and cellular biology. 1999;19:4935-43.

PubMed: 10373543 · PMC: PMC84302


Cullin 4A associates with the UV-damaged DNA-binding protein DDB. Shiyanov P, Nag A, Raychaudhuri P. The Journal of biological chemistry. 1999;274:35309-12.

PubMed: 10585395

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