LSBio
IHC-plus™ Polyclonal Rabbit anti-Human CUL5 / Cullin-5 Antibody (aa1-25, IHC, WB)

Anti-CUL5 / Cullin 5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ Polyclonal Rabbit anti-Human CUL5 / Cullin-5 Antibody (aa1-25, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B361-50

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Overview

Description
Cullin-5 antibody LS-B361 is an unconjugated rabbit polyclonal antibody to human Cullin-5 (CUL5) (aa1-25). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CUL5 | Cullin 5 | CUL-5 | VACM-1 | VACM1 | Cullin-5
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q93034
SwissProt
Q93034

Target

Target
CUL5 / Cullin-5
Antigen Species
Human
Specificity
Reacts with human Cullin 5. Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
Epitope
Reacts with human Cullin 5. Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
Gene Name
CUL5

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 1-25 of Human Cul5 (N-terminus) coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.

cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM. Cell. 1996;85:829-39.

PubMed: 8681378


ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999;3:535-41.

PubMed: 10230407

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