LSBio
IHC-plus™ Polyclonal Rabbit anti-Human ANAPC1 / APC1 Antibody (phospho-Ser377, IHC, WB)

Anti-ANAPC1 antibody IHC of human pancreatic carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human ANAPC1 / APC1 Antibody (phospho-Ser377, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B68-50

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Overview

Description
APC1 antibody LS-B68 is an unconjugated rabbit polyclonal antibody to human APC1 (ANAPC1) (pSer377). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Dog, Chicken, Mouse, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 | ANAPC1 | Cyclosome subunit 1 | MCPR | Mitotic checkpoint regulator | TSG24
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:200 - 1:1000
UniProt Number
Q9H1A4
SwissProt
Q9H1A4

Target

Target
ANAPC1 / APC1
Antigen Species
Human
Specificity
This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Epitope
This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Gene Name
ANAPC1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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