LSBio
IHC-plus™ Polyclonal Rabbit anti-Bovine S100 Protein Antibody (IHC, WB)

Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Bovine S100 Protein Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine
Format:Lyophilized
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$546.00each
Catalog Number: LS-B47-50

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Overview

Description
S100 Protein antibody LS-B47 is an unconjugated rabbit polyclonal antibody to bovine S100 Protein. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Bov
Predicted Reactivity
Dog, Mouse, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:500 - 1:3000

Target

Target
S100 Protein
Antigen Species
Bovine
Specificity
A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.