LSBio
IHC-plus™ Polyclonal Goat anti-Human NODAL Antibody (aa242-253, IHC, WB)

Anti-NODAL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

IHC-plus™ Polyclonal Goat anti-Human NODAL Antibody (aa242-253, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Dog, Horse, Human, Mammal (Other), Non-Human Primate, Pig
Format:Liquid
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$383.00each
Catalog Number: LS-B3955-50

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Overview

Description
NODAL antibody LS-B3955 is an unconjugated goat polyclonal antibody to NODAL (aa242-253) from human. It is reactive with human, bat, bovine and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Bov, Can, Hr, Hu, Mml, NHP, Por
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HTX5 | Nodal homolog | Nodal, mouse, homolog | NODAL | Nodal homolog (mouse)
Recommended Dilution: IHC-P
3.75 µg/ml
Recommended Dilution: WB
0.01 - 0.1 µg/ml
UniProt Number
Q96S42
SwissProt
Q96S42

Target

Target
NODAL
Antigen Species
Human
Specificity
Human NODAL.
Epitope
Human NODAL.
Gene Name
NODAL

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence DRSQLCRKVKFQ, from the internal region of the protein sequence according to NP_060525.2.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Strizzi L, Sandomenico A, Margaryan NV, Focà A, Sanguigno L, Bodenstine TM, Chandler GS, Reed DW, Gilgur A, Seftor EA, Seftor RE, Khalkhali-Ellis Z, Leonardi A, Ruvo M, Hendrix MJ. Oncotarget. 2015;6:34071-86.

PubMed: 26460952 · PMC: PMC4741437


Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Margaryan NV, Gilgur A, Seftor EA, Purnell C, Arva NC, Gosain AK, Hendrix MJ, Strizzi L. International journal of molecular sciences. 2016;17:418.

PubMed: 27011171 · PMC: PMC4813269


Targeting melanoma with front-line therapy does not abrogate Nodal-expressing tumor cells. Mary Jc Hendrix, Irawati Kandela, Andrew P Mazar, Elisabeth A Seftor, Richard Eb Seftor, Naira V Margaryan, Luigi Strizzi, George F Murphy, Georgina V Long, Richard A Scolyer. Laboratory investigation; a journal of technical methods and pathology. 2017 February;97:176-186.

PubMed: 27775691

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