LSBio
IHC-plus™ Polyclonal Goat anti-Human CD14 Antibody (aa71-84, IHC, WB)

Anti-CD14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.

IHC-plus™ Polyclonal Goat anti-Human CD14 Antibody (aa71-84, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B3012-50

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Overview

Description
CD14 antibody LS-B3012 is an unconjugated goat polyclonal antibody to human CD14 (aa71-84). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD14 molecule | CD14 antigen | CD14
Recommended Dilution: IHC-P
4 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
P08571
SwissProt
P08571

Target

Target
CD14
Antigen Species
Human
Specificity
aa71-84 of human, gorilla, orangutan CD14
Epitope
Human CD14.
Gene Name
CD14

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

Control of tumor-associated macrophages and T cells in glioblastoma via AHR and CD3910<sup> Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. fquintana@rics.bwh.harvard.edu. Maisa C Takenaka, Galina Gabriely, Veit Rothhammer, Ivan D Mascanfroni, Michael A Wheeler, Chun-Cheih Chao, Cristina Gutiérrez-Vázquez, Jessica Kenison, Emily C Tjon, Andreia Barroso, Tyler Vandeventer, Kalil Alves de Lima, Sonja Rothweiler, Lior Mayo, Soufiene Ghannam, Stephanie Zandee, Luke Healy, David Sherr, Mauricio F Farez , Alexandre Prat, Jack Antel, David A Reardon, Hailei Zhang, Simon C Robson, Gad Getz, Howard L Weiner, Francisco J Quintana. Nature neuroscience. 2019 May;;22:729-740.

PubMed: 30962630 · PMC: PMC8052632


P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Alexander Mildner, Hao Huang, Josefine Radke, Werner Stenzel, Josef Priller. Glia. 2017 Feburary;65:375-387.

PubMed: 27862351

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