Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ Polyclonal Goat anti-Human BMI1 / PCGF4 Antibody (IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B346-50
Catalog Number: LS-B346
For bulk orders or custom formulations:
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- Description
- PCGF4 antibody LS-B346 is an unconjugated goat polyclonal antibody to human PCGF4 (BMI1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Predicted Reactivity
- Bovine, Dog, Human, Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
- Recommended Dilution: ELISA
- 1:5000 - 1:30000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC-P
- 2.5 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P35226
- NCBI GenBank
- NM_005180 | NP_005171.4
- SwissProt
- P35226
Target
- Target
- BMI1 / PCGF4
- Antigen Species
- Human
- Specificity
- A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.
- Epitope
- A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.
- Gene Name
- BMI1 | COMMD3-BMI1
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.91 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
