LSBio
IHC-plus™ PIK3CA / PI3K Alpha Antibody (Internal)

Anti-PIK3CA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PIK3CA / PI3K Alpha Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Rat
Format:Liquid
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$495.00each
Catalog Number: LS-B5363-50

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Overview

Description
PI3K Alpha antibody LS-B5363 is an unconjugated rabbit polyclonal antibody to PI3K Alpha (PIK3CA) (Internal) from human. It is reactive with human and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
MCAP | p110-alpha | p110alpha | PI3K | PI3-kinase p110 subunit alpha | PI3-kinase subunit alpha | PI3Kalpha | PIK3CA | MCM | MCMTC | PI3K-alpha | PtdIns-3-kinase subunit alpha | CLOVE
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:2000
UniProt Number
P42336
SwissProt
P42336

Target

Target
PIK3CA / PI3K Alpha
Antigen Species
Human
Specificity
Recognizes human PI3 Kinase p110 alpha. Species cross-reactivity: rat.
Epitope
Recognizes human PI3 Kinase p110 alpha. Species cross-reactivity: rat.
Gene Name
PIK3CA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic linear peptide corresponding to an internal region of human class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform (KLH).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Recommended Storage Buffer
0.1 M Tris-Glycine, 150 mM NaCl
Epidermal growth factor receptor pathway mutation and expression profiles in cervical squamous cell carcinoma: therapeutic implications. Bumrungthai S, Munjal K, Nandekar S, Cooper K, Ekalaksananan T, Pientong C, Evans MF. Journal of translational medicine. 2015 July;13. PubMed: 26209091 PMC: PMC4513684

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