LSBio
IHC-plus™ PDIA3 / ERp57 Antibody (C-Terminus)

Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PDIA3 / ERp57 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Dog, Human, Mouse, Non-Human Primate, Rat
Format:Liquid
See all PDIA3 / ERp57 antibodies →
$470.00each
Catalog Number: LS-B9768-100

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Overview

Description
ERp57 antibody LS-B9768 is an unconjugated goat polyclonal antibody to ERp57 (PDIA3) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
IF, IHC, IHC-P, WB
Reactivity
Can, Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
58 kDa microsomal protein | ER protein 57 | ERp60 | ERp61 | Disulfide isomerase ER-60 | Endoplasmic reticulum P58 | GRP58 | HsT17083 | p58 | PDIA3 | Phospholipase C-alpha | PI-PLC | ER protein 60 | ER60 | ERp57 | GRP57 | Protein disulfide-isomerase A3
Recommended Dilution: IF/ICC
1:50 - 1:500
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P30101
SwissProt
P30101

Target

Target
PDIA3 / ERp57
Antigen Species
Human
Specificity
C-terminus of ERp57 from human, mouse, rat and other mammals
Epitope
Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.
Gene Name
PDIA3

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Purified recombinant peptide derived from within residues 300 aa to the C-Terminus of human ERP57 produced in E. coli.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide, 20% Glycerol
Concentration
3 mg/mL
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Chamberlain N, Korwin-Mihavics BR, Nakada EM, Bruno SR, Heppner DE, Chapman DG, Hoffman SM, van der Vliet A, Suratt BT, Dienz O, Alcorn JF, Anathy V. Redox biology. 2019 April;22:101129.

PubMed: 30735910 · PMC: PMC6365984


Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung. Nicolas Chamberlain, Mona Ruban, Zoe F Mark, Sierra R Bruno, Amit Kumar, Ravishankar Chandrasekaran, Dhemerson Souza De Lima, Danielle Antos, Emily M Nakada, John F Alcorn, Vikas Anathy. International journal of molecular sciences. 2022 January;23:1078.

PubMed: 35162999 · PMC: PMC8834910

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