LSBio
IHC-plus™ p16INK4a / CDKN2A Antibody

Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.

IHC-plus™ p16INK4a / CDKN2A Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$556.00each
Catalog Number: LS-B1347-0.05

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Overview

Description
CDKN2A antibody LS-B1347 is an unconjugated rabbit polyclonal antibody to CDKN2A (p16INK4a) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
INK4A | Multiple tumor suppressor 1 | MTS-1 | MTS1 | p16-INK4A | p16 INK4A | p16INK4A | CDKN2A | cyclin-dependent kinase inhibitor 2A
Recommended Dilution: IF/ICC
20 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 4 µg/ml

Target

Target
p16INK4a / CDKN2A
Antigen Species
Human

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
An 18 amino acid synthetic peptide near the N-Terminus of human CDKN2A. The immunogen is located within the first 50 amino acids of CDKN2A.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS containing 0.02% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. Zhang Y, Reddy A, Mohammed A, Lightfoot S, Dai W, Rao CV. Carcinogenesis. Mar.

PubMed: 25740822


Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. Sanghera S, Zhang Y, Biddick L, Reddy A, Lightfoot S, Dai W, Yamada HY. Molecular carcinogenesis. 2015 Mar.

PubMed: 25773652


Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Yamano S, Gi M, Tago Y, Doi K, Okada S, Hirayama Y, Tachibana H, Ishii N, Fujioka M, Tatsumi K, Wanibuchi H. Cancer science. 2016 February;107:123-32.

PubMed: 26663681 · PMC: PMC4768398


Delayed notochordal cell disappearance through integrin a5ß1 mechanotransduction during ex-vivo dynamic loading-induced intervertebral disc degeneration. Yutaro Kanda, Takashi Yurube, Yusuke Morita, Yoshiki Takeoka, Takuto Kurakawa, Ryu Tsujimoto, Kunihiko Miyazaki, Yuji Kakiuchi, Shingo Miyazaki, Zhongying Zhang, Toru Takada, Yuichi Hoshino, Koichi Masuda, Ryosuke Kuroda, Kenichiro Kakutani. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2021 September;39:1933-1944.

PubMed: 33049071

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