NES / Nestin Antibody
Catalog Number: LS-C137454-100
100 µL (1 mg/mL) / $295.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ NES / Nestin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B656-50
Catalog Number: LS-B656
For bulk orders or custom formulations:
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- Description
- Nestin antibody LS-B656 is an unconjugated rabbit polyclonal antibody to Nestin (NES) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Ms
- Predicted Reactivity
- Human, Non-Human Primate, Rabbit
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NES | Nestin | Nbla00170
- Recommended Dilution: ELISA
- 1:20000 - 1:80000
- Recommended Dilution: IHC
- 5 µg/ml
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P48681
- NCBI GenBank
- CAA46780.1 | X65964
- SwissProt
- P48681
Target
- Target
- NES / Nestin
- Antigen Species
- Human
- Specificity
- A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.
- Epitope
- A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.
- Gene Name
- NES
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.23 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
