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IHC-plus™ Monoclonal Rat anti-Human AHSA1 / AHA1 Antibody (clone 25F2.D9, IHC, WB)

Anti-AHA1 Monoclonal Antibody - Immunohistochemistry. anti-AHA1 monoclonal antibody was used at a 5-10 ug/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. expression of AHA1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.

IHC-plus™ Monoclonal Rat anti-Human AHSA1 / AHA1 Antibody (clone 25F2.D9, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B10601-50

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Overview

Description
AHA1 antibody LS-B10601 is an unconjugated rat monoclonal antibody to AHA1 (AHSA1) from human. It is reactive with human, mouse and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Non-Human Primate
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
AHA1 | AHSA1 | C14orf3 | p38
Recommended Dilution: ELISA
1:20000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
O95433
SwissProt
O95433

Target

Target
AHSA1 / AHA1
Antigen Species
Human
Specificity
This antibody is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Epitope
This antibody is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
AHSA1

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
25F2.D9
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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