LSBio
IHC-plus™ Monoclonal Rabbit anti-Human PARP1 Antibody (clone 46D11, Gly623, IHC, IF, WB)

Anti-PARP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC-plus™ Monoclonal Rabbit anti-Human PARP1 Antibody (clone 46D11, Gly623, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Non-Human Primate, Rat
Format:Liquid
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$577.00each
Catalog Number: LS-B5991-50

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Overview

Description
PARP1 antibody LS-B5991 is an unconjugated rabbit monoclonal antibody to PARP1 (Gly623) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ADPRT | ADP-ribosyltransferase NAD(+) | Adp-ribosyltransferase | ADPRT1 | ARTD1 | ADPRT 1 | Poly(ADP-ribose) synthetase | Poly(ADP-ribosyl)transferase | PARP | Poly [ADP-ribose] polymerase 1 | PADPRT-1 | PARP-1 | PARP1 | Poly (ADP-ribose) polymerase 1 | Poly(ADP-ribose) polymerase | Poly[ADP-ribose] synthase 1 | PPOL
Recommended Dilution: IF/ICC
1:400
Recommended Dilution: IHC-P
1:50
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:1000
UniProt Number
P09874
SwissProt
P09874

Target

Target
PARP1
Antigen Species
Human
Specificity
Detects endogenous levels of total full-length PARP and the large fragment (89kD) produced by caspase cleavage.
Epitope
Detects endogenous levels of total full-length PARP and the large fragment (89kD) produced by caspase cleavage.
Gene Name
PARP1

Antibody

Host Species
Rabbit
Clonality
Monoclonal
Clone
46D11
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide (KLH coupled) corresponding to residues surrounding Gly623 of PARP.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
Volume
50 Microliter
Animal Free
No
Carrier-Free
No
Preservative
Yes

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES, 150 mM sodium chloride

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.