LSBio
IHC-plus™ Monoclonal Mouse anti-Human THBS1 / Thrombospondin-1 Antibody (clone A6.1, Azide-free, IHC, IF, WB)

Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

IHC-plus™ Monoclonal Mouse anti-Human THBS1 / Thrombospondin-1 Antibody (clone A6.1, Azide-free, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Dog, Horse, Human, Mouse, Pig, Rat, Sheep
Format:Liquid
See all THBS1 / Thrombospondin-1 antibodies →
$495.00each
Catalog Number: LS-B2570-50

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Overview

Description
Thrombospondin-1 antibody LS-B2570 is an unconjugated mouse monoclonal antibody to Thrombospondin-1 (THBS1) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
FC, IF, IHC, IHC-P, IP, WB
Reactivity
Bov, Can, Hr, Hu, Ms, Por, Rt, Sh
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
THBS1 | Thrombospondin-1 | Thrombospondin-1p180 | TSP | TSP1 | THBS-1 | THBS | Thrombospondin 1 | TSP-1
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P07996
SwissProt
P07996

Target

Target
THBS1 / Thrombospondin-1
Antigen Species
Human
Specificity
Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
Epitope
Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
Gene Name
THBS1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
A6.1
Origin Species
Human

Immunogen

Immunogen
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP
Immunogen Type
Denatured Protein / SDS-PAGE Band

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.