LSBio
IHC-plus™ Monoclonal Mouse anti-Human SKP2 Antibody (IHC, WB)

Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human SKP2 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2487-50

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Overview

Description
SKP2 antibody LS-B2487 is an unconjugated mouse monoclonal antibody to human SKP2. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
F-box/LRR-repeat protein 1 | FBL1 | FLB1 | F-box protein Skp2 | FBXL1 | p45 | p45skp2 | SKP2
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 2 µg/ml
UniProt Number
Q13309
SwissProt
Q13309

Target

Target
SKP2
Antigen Species
Human
Specificity
Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.
Epitope
Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.
Gene Name
SKP2

Antibody

Host Species
Mouse
Clonality
Monoclonal
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Recombinant, full-length human p45SKP2 protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Damage-induced BRCA1 phosphorylation by Chk2 contributes to the timing of end resection. Parameswaran B, Chiang HC, Lu Y, Coates J, Deng CX, Baer R, Lin HK, Li R, Paull TT, Hu Y. Cell cycle (Georgetown, Tex.). 2015;14:437-48.

PubMed: 25659039


Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop. Leilei Qi, Reka Chakravarthy, Monica M Li, Chu-Xia Deng, Rong Li, Yanfen Hu. iScience. 2022 September;25:104944.

PubMed: 36065181 · PMC: PMC9440284

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