LSBio
IHC-plus™ Monoclonal Mouse anti-Human NKX3-1 Antibody (clone 3-9, N-Terminus, IHC, WB)

Anti-NKX3-1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human NKX3-1 Antibody (clone 3-9, N-Terminus, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B3435-50

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Overview

Description
NKX3-1 antibody LS-B3435 is an unconjugated mouse monoclonal antibody to human NKX3-1 (N-Terminus). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BAPX2 | NK homeobox, family 3, A | NK3 homeobox 1 | NKX3 | NKX3-1 | NKX3.1 | Homeobox protein Nkx-3.1 | NKX3A
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
Q99801
SwissProt
Q99801

Target

Target
NKX3-1
Antigen Species
Human
Specificity
Reacts with human Nkx3.1 protein, and does not cross-react with mouse. On Western blots, this antibody detects a band at ~34kD. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.
Epitope
Reacts with human Nkx3.1 protein, and does not cross-react with mouse. On Western blots, this antibody detects a band at ~34kD. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.
Gene Name
NKX3-1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
3-9
Origin Species
Human

Immunogen

Immunogen
Fusion protein containing the N-Terminus domain of the human Nkx3.1
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C.
Recommended Storage Buffer
PBS
NKX3.1 haploinsufficiency is prognostic for prostate cancer relapse following surgery or image-guided radiotherapy. Locke JA, Zafarana G, Ishkanian AS, Milosevic M, Thoms J, Have CL, Malloff CA, Lam WL, Squire JA, Pintilie M, Sykes J, Ramnarine VR, Meng A, Ahmed O, Jurisica I, van der Kwast T, Bristow RG. Clinical cancer research : an official journal of the American Association for Cancer Research. 2014 Jan;18:308-16. PubMed: 22048240

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