LSBio
IHC-plus™ Monoclonal Mouse anti-Human Muscle Actin Antibody (clone HHF35, IHC, WB)

Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ Monoclonal Mouse anti-Human Muscle Actin Antibody (clone HHF35, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunohistochemistry (general)
Reactivity:Chicken, Human, Non-Human Primate, Pig, Rabbit, Rat, Zebrafish
Format:Liquid
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$383.00each
Catalog Number: LS-B8464-50

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Overview

Description
Muscle Actin antibody LS-B8464 is an unconjugated mouse monoclonal antibody to Muscle Actin from human. It is reactive with human, rat, chicken and other species. Validated for ELISA, EM, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
IHC
Reactivity
Ck, Hu, NHP, Por, Rb, Rt, Zf
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
MSA | Muscle Specific Actin | HHF35
Recommended Dilution: IHC-F
1:200 - 1:1000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Muscle Actin
Antigen Species
Human
Specificity
Reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. The epitope recognized by HHF35 is highly conserved. Therefore the antibody cross-reacts with several species including human, monkey, swine, rabbit, rat, chicken and zebrafish
Epitope
Reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. The epitope recognized by HHF35 is highly conserved. Therefore the antibody cross-reacts with several species including human, monkey, swine, rabbit, rat, chicken and zebrafish

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
HHF35

Immunogen

Immunogen
SDS-extracted protein fraction from human myocardium

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C. Avoid freezing.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.