LSBio
IHC-plus™ Monoclonal Mouse anti-Human HSP70 / Heat Shock Protein 70 Antibody (clone 5A5, IHC, IF, WB)

Anti-HSP70 / Heat Shock Protein 70 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Monoclonal Mouse anti-Human HSP70 / Heat Shock Protein 70 Antibody (clone 5A5, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Chicken, Fish (Other), Fruit Fly, Human, Mouse, Other, Rat
Format:Liquid
See all HSP70 / Heat Shock Protein 70 antibodies →
$383.00each
Catalog Number: LS-B7451-50

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Overview

Description
Heat Shock Protein 70 antibody LS-B7451 is an unconjugated mouse monoclonal antibody to Heat Shock Protein 70 (HSP70) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IF, IHC, IHC-P, IP, WB
Reactivity
Ck, Dros, Fish, Hu, Ms, Other, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IF/ICC
1:500
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000

Target

Target
HSP70 / Heat Shock Protein 70
Antigen Species
Human
Specificity
Detects several members of the heat shock protein 70kD gene family including Hsp70, Hsc70, Grp78, and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.
Epitope
Detects several members of the heat shock protein 70kD gene family including Hsp70, Hsc70, Grp78, and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
5A5

Immunogen

Immunogen
Human Recombinant Hsp70 overexpressed in E. coli.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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