LSBio
IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6)

Anti-MHC Class II RT-1B antibody IHC of rat spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Rat
Format:Liquid
See all MHC Class II RT-1B antibodies →
$577.00each
Catalog Number: LS-B5258-0.25

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Overview

Description
MHC Class II RT-1B antibody LS-B5258 is an unconjugated mouse monoclonal antibody to rat MHC Class II RT-1B. Validated for Flow, IF and IHC.
Application
FC, IF, IHC, IHC-F, IHC-P
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:50 - 1:100
Recommended Dilution: IHC-P
10 µg/ml

Target

Target
MHC Class II RT-1B
Antigen Species
Rat
Specificity
Recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression. also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.
Epitope
Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OX-6

Immunogen

Immunogen
Rat thymocyte membrane glycoprotein.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.